ratkin412
Well-Known Member
From the study
"At M38 all water-treated control rats were dead (Fig. 3a). This agrees with the expected lifespan of this animal species that is thirty to thirty six months. At this time 67% of olive-oil-treated rats and 100% of C60-treated rats were still alive. "
Here is my compliation of the pertinent areas of the study dealing with longevity...
Basically, it boils down to this:
They took a bunch of 10 month old male Wister rats and put them on dosages of 1.7 mg per kg of body weight - the 'medicine' consisted of .8 mg C-60 per ml of olive oil, in a suspension. They dosed the rats daily for one week, then weekly until the end of the second month, then every two weeks until the end of the 7th month. The treatment was stopped when a control rat died at 17 months old. By month 38, all of the control rats had died, but all of the C-60 rats were still alive. They estimated the balance of the C-60 rat's lives with a formula designed to do so.
The above information can be found in detail under the spoiler:
: [spoil:1jvwvnw9]From the 2012 Study results: The prolongation of the lifespan of rats by repeated oral administration of [60]
Fullerene - : Baati T, et al., The prolongation of the lifespan of rats by repeated oral administration of [60]fullerene,
Biomaterials (2012), doi:10.1016/j.biomaterials.2012.03.036
http://extremelongevity.net/wp-content/ ... lerene.pdf
Rats:
Ten-month old male rats (M10) were chosen instead of young rats as ofï¬cially recommended [27], in order to avoid possible compensatory effects that can occur during early development [44]. As biodistribution studies after daily gavages showed that C60 accumulates in livers and spleens, in order to avoid the negative effects of prolonged olive oil administration such as obesity, excessive steatosis, liver lipid degeneration, and insulin resistance [45], we treated the rats daily only during 7 days and weekly during the ï¬rst two months, then every two weeks until one control rat died. Our results show that while olive oil treatment can lead to an increase of 18% of lifespan of treated rats, C60-olive oil can increase it up to 90%, as compared to controls. The effects of olive-oil on health and ageing are well known [46], and its effect as a function of dose has been thoroughly discussed [45]. But, what is noteworthy is
that at M38 all C60-treated rats were still alive. Thus, based on previous investigations [44], C60 should be the most efï¬cient ever material for extending lifespan⦠Pharmacokinetic studies were carried out with male Wistar rats (weighing 200e220 g). Rats were housed in individual cages and maintained in an airconditioned room (22e25
C) on a 12 h light/dark cycle with water and food available. The rats were acclimated for 7 days before treatment.
Dosage: oral administration of C60 dissolved in olive oil (0.8 mg/ml) at reiterated doses (1.7 mg/kg of body weight) to rats not only does not entail chronic toxicity but it almost doubles their lifespan. (Virgin olive oil is obtained from a Chemlali Boughrara cultivar from Tunisia planted in the Sahel area. C60 (purity 99.98%) was obtained from SES Research Corporation (USA) and used without further puriï¬cation. Fifty mg of C60 were dissolved in 10 ml of olive oil by stirring for 2 weeks at ambient temperature in the dark. The resulting mixture was centrifuged at 5.000 g for 1 h and the supernatant was ï¬ltered through a Millipore ï¬lter with 0.25 mm porosity⦠The resulting C60-olive oil solution is purple and contains 0.80 0.02 mg/ml (n ¼ 6) as determined by HPLC [30] after appropriate dilution in the mobile phase. The chromatographic proï¬le and the extracted spectra of these solutions are similar to those obtained with a control C60-toluene equimolar solution. The stability of both oily and control solutions stored at ambient temperature and in the dark was checked monthly during 48 months. No change was recorded under our chromatographic conditions.) â¦4.1. C60-olive oil solution preparation It is well known that C60 and derivatives are prone to aggregate even in their best solvents [37]. The C60-olive oil solution used in this study can be considered as free of C60 aggregates because: 1 e its colour is purple that is characteristic of C60 solutions while the colour of C60 aggregate-containing solutions are rather brown, which is true even for water-soluble derivatives [3]; 2 e it is freely
and instantaneously soluble in toluene in contrast to C60 aggregatecontaining solutions, which slowly dissolve even in the best solvents of C60. Besides, the concentrations of C60 in olive oil as determined by HPLC agree with those previously published by other authors [22]. The stability of C60-olive oil solution determined under our experimental conditions agrees with recently published result showing that the addition of [60]fullerene signiï¬cantly hampers the peroxide formation thus increasing the stability of the tested oils [38]â¦. It is to be stressed that dissolved C60 appears hundred times more active than when it is in suspension [21]. In fact the action of soluble C60 is immediate while that of suspended C60 is delayed because it has to be dissolved to act.
Medication timeline:
The rats were housed three per cage and acclimated for 14 days, before dosing. Three groups of 6 rats (10 months old, weighing 465 31 g) were administered daily for one week, then weekly until the end of the second month and then every two weeks until the end of the 7th month, by gavages with 1 ml of water or olive oil or C60 dissolved in olive oil (0.8 mg/ml), respectively
The survival distributions for C60-olive oil-treated and control rats were estimated by the non-parametric KaplaneMeier estimator and compared by a log-rank estimated test.
These results obtained with a small sample of animals with an exploratory protocol ask for a more extensive studies to optimize the intestinal absorption of C60 as well as the different parameters of the administration protocol: dose, posology, and treatment duration. In the present case, the treatment was stopped when a control rat died at M17, which proves that the effects of the C60 treatment are long-lasting as the estimated median lifespan for C60-treated rats is 42 months. It can be thought that a longer treatment could have generated even longer lifespans.
3.3. Chronic toxicity and effects of C60 on lifespan of ratsFig. 3 shows the animal survival and growth. After ï¬ve months of treatment (M15) one rat treated with water only exhibited some palpable tumours in the abdomen region. Due to the rapid development of tumours (about 4 cm of diameter) this rat died at M17. As rats are known to be sensitive to gavages, we decided to stop the treatment for all rats and to observe their behaviour and overall survival.
All remaining animals survived with no apparent sign of behavioural trouble until M25 (Fig. 3a). At the end of M25 the animals of the control groups showed signs of ulcerative dermatitis with ageing while C60-treated animals remained normal. As the growths of all surviving animals showed no signiï¬cant difference until M30 (Fig. 3b) indicating that the treatment did not alter their food intake, we continued observing their survival. At M38 all water-treated control rats were dead (Fig. 3a). This agrees with the expected lifespan of this animal species that is thirty to thirty six months. At this time 67% of olive-oil-treated rats and 100% of C60-treated rats were still alive. The survival distributions for C60-olive oil-treated rats and controls were estimated by the non-parametric KaplaneMeier estimator (Fig. 3) and compared by a log-rank estimated test. The estimated median lifespan (EML) for the C60-treated rats was 42 months while the EMLs for control rats and olive oil-treated rats were 22 and 26 months, respectively. These are increases of 18 and 90% for the olive-oil and C60-treated rats, respectively, as compared to controls. The log-rank test leads to c 2 values (one degree of freedom) of 7.009, 11.302, and 10.454, when we compare water-treated and olive oil-treated rats, water-treated and C60-treated rats, and olive oil-treated and C60-treated rats, respectively. This means that olive oil extends the lifespan of rats with respect to water with a probability of 0.99 while C60-olive oil extends the lifespan of C60-treated rats with a probability of 0.999 and 0.995 with respect to water and olive oil treatments, respectively
Notes;
Signiï¬cantly weaker similar effects have already been reported in several experimental models but for different hydrosoluble C60- derivatives [44,47]. The effects of C60-derivatives on ageing were attributed to the antioxidant properties and the attenuation of ageassociated increases in oxidative stress [4,44[/spoil:1jvwvnw9]
I am very optimistic about this study, and the results, although I can't help but feel that these sorts of studies are immoral and horrible.
"At M38 all water-treated control rats were dead (Fig. 3a). This agrees with the expected lifespan of this animal species that is thirty to thirty six months. At this time 67% of olive-oil-treated rats and 100% of C60-treated rats were still alive. "
Here is my compliation of the pertinent areas of the study dealing with longevity...
Basically, it boils down to this:
They took a bunch of 10 month old male Wister rats and put them on dosages of 1.7 mg per kg of body weight - the 'medicine' consisted of .8 mg C-60 per ml of olive oil, in a suspension. They dosed the rats daily for one week, then weekly until the end of the second month, then every two weeks until the end of the 7th month. The treatment was stopped when a control rat died at 17 months old. By month 38, all of the control rats had died, but all of the C-60 rats were still alive. They estimated the balance of the C-60 rat's lives with a formula designed to do so.
The above information can be found in detail under the spoiler:
: [spoil:1jvwvnw9]From the 2012 Study results: The prolongation of the lifespan of rats by repeated oral administration of [60]
Fullerene - : Baati T, et al., The prolongation of the lifespan of rats by repeated oral administration of [60]fullerene,
Biomaterials (2012), doi:10.1016/j.biomaterials.2012.03.036
http://extremelongevity.net/wp-content/ ... lerene.pdf
Rats:
Ten-month old male rats (M10) were chosen instead of young rats as ofï¬cially recommended [27], in order to avoid possible compensatory effects that can occur during early development [44]. As biodistribution studies after daily gavages showed that C60 accumulates in livers and spleens, in order to avoid the negative effects of prolonged olive oil administration such as obesity, excessive steatosis, liver lipid degeneration, and insulin resistance [45], we treated the rats daily only during 7 days and weekly during the ï¬rst two months, then every two weeks until one control rat died. Our results show that while olive oil treatment can lead to an increase of 18% of lifespan of treated rats, C60-olive oil can increase it up to 90%, as compared to controls. The effects of olive-oil on health and ageing are well known [46], and its effect as a function of dose has been thoroughly discussed [45]. But, what is noteworthy is
that at M38 all C60-treated rats were still alive. Thus, based on previous investigations [44], C60 should be the most efï¬cient ever material for extending lifespan⦠Pharmacokinetic studies were carried out with male Wistar rats (weighing 200e220 g). Rats were housed in individual cages and maintained in an airconditioned room (22e25
C) on a 12 h light/dark cycle with water and food available. The rats were acclimated for 7 days before treatment.
Dosage: oral administration of C60 dissolved in olive oil (0.8 mg/ml) at reiterated doses (1.7 mg/kg of body weight) to rats not only does not entail chronic toxicity but it almost doubles their lifespan. (Virgin olive oil is obtained from a Chemlali Boughrara cultivar from Tunisia planted in the Sahel area. C60 (purity 99.98%) was obtained from SES Research Corporation (USA) and used without further puriï¬cation. Fifty mg of C60 were dissolved in 10 ml of olive oil by stirring for 2 weeks at ambient temperature in the dark. The resulting mixture was centrifuged at 5.000 g for 1 h and the supernatant was ï¬ltered through a Millipore ï¬lter with 0.25 mm porosity⦠The resulting C60-olive oil solution is purple and contains 0.80 0.02 mg/ml (n ¼ 6) as determined by HPLC [30] after appropriate dilution in the mobile phase. The chromatographic proï¬le and the extracted spectra of these solutions are similar to those obtained with a control C60-toluene equimolar solution. The stability of both oily and control solutions stored at ambient temperature and in the dark was checked monthly during 48 months. No change was recorded under our chromatographic conditions.) â¦4.1. C60-olive oil solution preparation It is well known that C60 and derivatives are prone to aggregate even in their best solvents [37]. The C60-olive oil solution used in this study can be considered as free of C60 aggregates because: 1 e its colour is purple that is characteristic of C60 solutions while the colour of C60 aggregate-containing solutions are rather brown, which is true even for water-soluble derivatives [3]; 2 e it is freely
and instantaneously soluble in toluene in contrast to C60 aggregatecontaining solutions, which slowly dissolve even in the best solvents of C60. Besides, the concentrations of C60 in olive oil as determined by HPLC agree with those previously published by other authors [22]. The stability of C60-olive oil solution determined under our experimental conditions agrees with recently published result showing that the addition of [60]fullerene signiï¬cantly hampers the peroxide formation thus increasing the stability of the tested oils [38]â¦. It is to be stressed that dissolved C60 appears hundred times more active than when it is in suspension [21]. In fact the action of soluble C60 is immediate while that of suspended C60 is delayed because it has to be dissolved to act.
Medication timeline:
The rats were housed three per cage and acclimated for 14 days, before dosing. Three groups of 6 rats (10 months old, weighing 465 31 g) were administered daily for one week, then weekly until the end of the second month and then every two weeks until the end of the 7th month, by gavages with 1 ml of water or olive oil or C60 dissolved in olive oil (0.8 mg/ml), respectively
The survival distributions for C60-olive oil-treated and control rats were estimated by the non-parametric KaplaneMeier estimator and compared by a log-rank estimated test.
These results obtained with a small sample of animals with an exploratory protocol ask for a more extensive studies to optimize the intestinal absorption of C60 as well as the different parameters of the administration protocol: dose, posology, and treatment duration. In the present case, the treatment was stopped when a control rat died at M17, which proves that the effects of the C60 treatment are long-lasting as the estimated median lifespan for C60-treated rats is 42 months. It can be thought that a longer treatment could have generated even longer lifespans.
3.3. Chronic toxicity and effects of C60 on lifespan of ratsFig. 3 shows the animal survival and growth. After ï¬ve months of treatment (M15) one rat treated with water only exhibited some palpable tumours in the abdomen region. Due to the rapid development of tumours (about 4 cm of diameter) this rat died at M17. As rats are known to be sensitive to gavages, we decided to stop the treatment for all rats and to observe their behaviour and overall survival.
All remaining animals survived with no apparent sign of behavioural trouble until M25 (Fig. 3a). At the end of M25 the animals of the control groups showed signs of ulcerative dermatitis with ageing while C60-treated animals remained normal. As the growths of all surviving animals showed no signiï¬cant difference until M30 (Fig. 3b) indicating that the treatment did not alter their food intake, we continued observing their survival. At M38 all water-treated control rats were dead (Fig. 3a). This agrees with the expected lifespan of this animal species that is thirty to thirty six months. At this time 67% of olive-oil-treated rats and 100% of C60-treated rats were still alive. The survival distributions for C60-olive oil-treated rats and controls were estimated by the non-parametric KaplaneMeier estimator (Fig. 3) and compared by a log-rank estimated test. The estimated median lifespan (EML) for the C60-treated rats was 42 months while the EMLs for control rats and olive oil-treated rats were 22 and 26 months, respectively. These are increases of 18 and 90% for the olive-oil and C60-treated rats, respectively, as compared to controls. The log-rank test leads to c 2 values (one degree of freedom) of 7.009, 11.302, and 10.454, when we compare water-treated and olive oil-treated rats, water-treated and C60-treated rats, and olive oil-treated and C60-treated rats, respectively. This means that olive oil extends the lifespan of rats with respect to water with a probability of 0.99 while C60-olive oil extends the lifespan of C60-treated rats with a probability of 0.999 and 0.995 with respect to water and olive oil treatments, respectively
Notes;
Signiï¬cantly weaker similar effects have already been reported in several experimental models but for different hydrosoluble C60- derivatives [44,47]. The effects of C60-derivatives on ageing were attributed to the antioxidant properties and the attenuation of ageassociated increases in oxidative stress [4,44[/spoil:1jvwvnw9]
I am very optimistic about this study, and the results, although I can't help but feel that these sorts of studies are immoral and horrible.
